Sharing advanced technology: core facilities in therapeutics

Therapeutics research requires robust resources for imaging, informatics, screening, chemistry and medical biochemistry. The Therapeutic Technology Cluster (TTC) is a component of HiTS still in development. The first new technology supported by the TTC is the Longwood Medical Area (LMA) CyTOF Core Facility. Support has also been provided to a number of existing cores to increase their capabilities.

Resources supported in part through the TTC

Mass Cytometry (highly multiplexed flow cytometry)
Longwood Medical Area (LMA) CyTOF Core Facility.  The heart of the LMA CyTOF Core Facility is a CyTOF®2 Mass Cytometer that is capable of analyzing, at the single cell level, up to 40 markers simultaneously with minimal signal overlap. The facility has both a dedicated operator and a data analyst who assist investigators as they design assays, process samples, and analyze the high dimensional data produced by the CyTOF.  The CyTOF Core Facility is a collaboration between HiTS, HMS, DFCI, BWH, BIDMC, HSCI and the Ragon Institute. For more information about the CyTOF Core Facility, please see the TTC LMA CyTOF Core Facility web page.

Small Molecule &RNAi Screening and Laboratory Automation
ICCB-Longwood Screening Facility (ICCB-L) provides resources and expertise for high throughput screening (HTS) of small molecule and genome-scale RNAi libraries. ICCB-L also provides general laboratory automation support for the HMS community.

Computational Analysis of Microscopy Data.
Image and Data Analysis Core (IDAC) provides advice and computing infrastructure for routine microscope image and data analysis, and provides computing as well as intellectual infrastructure for the development of new, customized image and data processing methods.  .

In vivo Luminescence or Fluorescence Imaging
The Mouse Imaging Core provides access for researchers to in-vivo mouse imaging for endpoint and longitudinal studies using either luminescent or fluorescent reporters in an Animal Biosafety Level 2 environment.   Staff from the ICCB-Longwood Screening Facility oversee the IVIS (In-Vivo Imaging System) Lumina II mouse imager and its use, while the IVIS room is operated and maintained by the HCCM (Harvard Center for Comparative Medicine).  Staff members provide access and training for the imager and data analysis computers, while the HCCM oversees all room and animal operations including facility access, safety training and orientation, animal transfers and husbandry, and protocol approval.

Center for Macromolecular Interactions
The CMI houses several shared instruments for the characterization of macromolecules and their interactions. The facility houses a Biacore T200, a MicroCal ITC-200, a Jasco J-815 CD, ForteBio BLItz and Octet instruments, an Agilent/Wyatt SEC-MALS system equipped with autosampler, an Applied Biosystems Quantstudio 6 thermocycler for differential scanning fluorimetry, and a preparative scale HPLC for denaturing purification of peptides and small proteins. For the booking system and rates, instrument sign-up and training requests, please follow the link here.